Research Solutions for 2019 Coronavirus SARS-CoV-2 (COVID-19)
The coronavirus disease outbreak that began in December 2019 is spreading rapidly throughout the world. The coronavirus strain was first identified in Wuhan, China, and has been named SARS-CoV-2 (the coronavirus responsible for COVID-19). The disease the virus causes is called COVID-19 and is characterized by fever, cough, and shortness of breath.
Researchers around the world are diligently working to gain a better understanding of the coronavirus with the goal of rapidly identifying future treatment options and possible vaccine targets. With a comprehensive portfolio of research products, Thermo Fisher Scientific is your trusted partner for pathogen detection, epidemiological surveillance, vaccine development, and the laboratory equipment needed to advance your research.
About Virus discovery
The Peter Doherty Institute for Infection and Immunity in Melbourne, Australia was the first scientific lab outside of China to sequence the 2019-nCoV genome. The team of researchers used the Applied Biosystems SeqStudio instrument to conduct sequencing to generate a reference sequence, which is fueling global surveillance and prevention efforts.
Video: Australian lab first outside of China to re-create coronavirus, helping vaccine development
Sequencing by capillary electrophoresis
In addition to its initial role in identification of SARS-CoV-2, Sanger sequencing by capillary electrophoresis can be used to confirm RT-PCR results and provide confidence distinguishing SARS-CoV-2 from other respiratory pathogens. In a simplified workflow, PCR product is purified and sequencing primers bind to known sequences in the target region thus allowing the sequence to be extended using capillary electrophoresis. For comprehensive information on sequencing using capillary electrophoresis refer to the Sequencing Chemistry Guide.
Whole-genome sequencing for discovery using NGS
In addition to targeted sequencing, which covers the complete viral genome and variants (see research surveillance tab), Ion Torrent next-generation sequencing (NGS) systems can be used for de novo (whole-genome) sequencing of a SARS-CoV-2.
